This project uses Hololens-based mixed reality technology to develop a printing press operation training system including interface, operation simulation, learning guidance, interaction and other modules. The system can guide the operator to the correct position of the real printing press and realize the virtual and real fusion of the printing press model and the real printing press. The simulation virtual model of printing machine carries out scientific and reasonable operation learning by combining natural interaction methods such as gaze, gesture and voice with guiding animation, demonstration video and explanation document, so as to avoid the wrong operation of operators and fundamentally improve the efficiency of operation training. According to the system design requirements, the system is divided into five process flows, such as plate loading, paper loading, water filling, ink filling and startup printing. Each process flow is also equipped with training process selection module, process flow working principle video, document demonstration module, process equipment structure learning module, equipment target tracking and registration module, specific operation step training module and so on.