With the diversified development of educational ideas in China, inquiry-based teaching focusing on personalized active learning and practical ability training has attracted more and more attention. Mixed reality technology provides the possibility of immersive virtual reality fusion experiment for inquiry learning. We have carried out an in-depth virtual-real fusion and media space design around the inquiry-based chemical experiment learning environment, combined with interactive projection, gesture interaction and intelligent hardware to design a virtual-real fusion of chemical experiment learning environment, so as to provide students with interesting chemical experiment interactive experience, but also for teachers to provide students with behavior tracking and experimental behavior visualization analysis. In this mixed reality media space, learners enter a chemistry laboratory with virtual and real fusion, and interact with virtual experimental props in the projection screen in real time through gestures to try infinite possibilities. Operate with real reagent bottles to experience the reality of scientific experiments; Observe experimental phenomena in intelligent beakers and experience the fun of inquiry. While observing students' behavior, teachers can easily obtain auxiliary information of students' body movements, historical records of operation behavior, behavior details and operation accuracy tips, etc., so as to realize inquiry teaching easily. This work provides a new, inquiry-based, virtual and real integration of teaching methods for secondary school chemistry experimental education. Compared with the traditional experimental teaching, the experimental environment of virtual and real integration has the advantages of full function, low cost, risk avoidance, breaking the limitation of space and time, and can enhance students' interest in learning and improve learning efficiency. At the same time, combined with the design of inquiry learning concept, teachers can also play a guiding role for students, so that teachers can give help and tips in the process of students' inquiry.